The Cl/OH initiated temperature dependent photo-oxidative reaction kinetics of methyl butyrate (MB) were examined using a relative rate (RR) technique. Gas chromatography with flame ionization and mass spectrometric detection were used to monitor the concentration of the reactants and to identify the products. The temperature dependent kinetics of MB with Cl atoms were measured with respect to the reaction of Cl with C2H 6 and C2H4. The temperature dependent kinetics for the reaction of MB with OH radicals were measured using n- propanol and iso-propanol as references. The obtained rate coefficients for the Cl and OH reactions with MB are, k Cl(Expt) (T) = [(7.76±0.47) × 10 -11] exp [(10.31±0.20)/T] cm3 molecule-1 s-1 and kOH(Expt) (T) = [(4.32±0.21) × 10 -12] exp [-(25.26±0.39)/T] cm3 molecule-1 s-1 respectively. Dual level direct dynamics were used to perform the computational calculations to further elucidate the mechanisms over the studied temperature range. The rate coefficients for Habstraction reactions were computed using Canonical Variational Transition State Theory with Small Curvature Tunneling (CVT/SCT) with Interpolated Single Point Energies (ISPE) method. The rate coefficients over the studied temperature range yielded the Arrhenius equations: k Cl(Theory) (200-400 K) = [(4.05±0.54) × 10-11] exp [-(2.80±0.11)/T] cm3 molecule-1 s-1 and k OH(Theory) (200-400 K) = [(1.96±0.68) × 10 -11 ] exp [-(384±38)/T] cm3 molecule -1 s -1. Possible degradation mechanisms for the reactions are proposed based on the Manuscript.